FOBI's functionality has been improved. The camera's sensitivity is improved about 3 times up to 10 times, and the excitation light source is also about up to 10 times stronger than before. In addition, FOBI 2 minimizes the interference of fluorescent images by changing the location of the light source (Light angle: 67º to 45º). Also, a heating bed is added to protect the experimental organisms from hypothermia.